公开(公告)号：US12054524B2

公开(公告)日：2024-08-06

申请号：US17692508

申请日：2022-03-11

Applicant: CHUNG-ANG UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Kwang Hoon Kong ,  Han Seul Kim ,  Si Wook Jang ,  Gi Hyeon Chae

IPC: C07K14/425 ,  C12N15/81

CPC classification number: C07K14/425 ,  C12N15/81 ,  C12N2800/102 ,  C12N2830/002 ,  C12N2830/704

Abstract: The present inventors confirmed that when a brazzein expression recombinant vector for high expression of brazzein in Saccharomyces cerevisiae was prepared and a S. cerevisiae strain Y2805 was transformed with the recombinant vector, the expression level of brazzein was particularly high, thereby completing an optimal expression system for mass-producing brazzein. Further, when the brazzein expression system is cultured under the optimal culture conditions according to the present invention, the amount of brazzein produced is further increased, the purification process is simple, and costs are reduced. Therefore, it is expected that the brazzein expression system according to the present invention can be widely used for mass-producing and commercializing brazzein, which is a sweet protein.