公开(公告)号：US20240310391A1

公开(公告)日：2024-09-19

申请号：US18615987

申请日：2024-03-25

Applicant: CLEVELAND HEARTLAB, INC.

Inventor： Cory Bystrom ,  Timothy COLLIER

IPC: G01N33/92

CPC classification number: G01N33/92 ,  G01N2333/775

Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.