公开(公告)号：US20230257822A1

公开(公告)日：2023-08-17

申请号：US17920931

申请日：2021-04-23

申请人： CORNELL UNIVERSITY

发明人： Iwijn DE VLAMINCK ,  Alexandre Pellan CHENG ,  Matthew Pellan CHENG ,  Francisco Miguel MARTY ,  Jerome RITZ

IPC分类号： C12Q1/6886 ,  C12Q1/6888

CPC分类号： C12Q1/6886 ,  C12Q1/6888 ,  C12Q2600/154

摘要： The present disclosure is directed to novel methods for detecting tissue damage, graft-versus-host disease (GVHD), microbial infections, presence of a tumor, and loss of engraftment in a subject using cell-free DNA (cfDNA) profiling. The methods of the disclosure are in part based on the recognition that damaged tissues, microbes during an infection, tumors, and donor cells (e.g., in a hematopoietic cell transplantation) shed small fragments of cfDNA into blood circulation.

公开(公告)号：US20240026456A1

公开(公告)日：2024-01-25

申请号：US18458199

申请日：2023-08-30

申请人： CORNELL UNIVERSITY

发明人： Iwijn DE VLAMINCK ,  John Richard LEE ,  Philip Smith BURNHAM ,  Alexandre Pellan CHENG ,  Manikkam SUTHANTHRIAN ,  Darshana M. DADHANIA

IPC分类号： C12Q1/6883 ,  C12Q1/689 ,  C12Q1/70 ,  C40B30/06

CPC分类号： C12Q1/6883 ,  C12Q1/689 ,  C12Q1/701 ,  C40B30/06 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present disclosure is directed to methods of detecting cell-free DNA (cfDNA) in biological samples and using it to quantify organ damage and identify pathogens. In some aspects, the biological samples are from patients who have undergone solid-organ transplantation. The disclosure is also directed to methods of detecting and analyzing methylation patterns in cell-free DNA from organ transplant patients to identify the presence of pathogens as well as quantify contributing tissue proportions as a measurement of the host response.

公开(公告)号：US20230323455A1

公开(公告)日：2023-10-12

申请号：US18017165

申请日：2021-07-23

申请人： CORNELL UNIVERSITY

发明人： Iwijn DE VLAMINCK ,  Alexandre Pellan CHENG ,  Matthew Pellan CHENG ,  Donald Cuong VINH ,  Wei GU ,  Charles CHIU

IPC分类号： C12Q1/70 ,  A61K31/573 ,  A61K31/664

CPC分类号： C12Q1/70 ,  A61K31/573 ,  A61K31/664 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present disclosure is directed to methods for assessing the severity and progression of a SARS-CoV2 infection in a subject using cell-free DNA (cfDNA). The present disclosure is further directed to methods for assessing tissue damage as a result of SARS-CoV2 infection. In addition, the present disclosure provides methods for treating a SARS-CoV2 infection in a subject. Finally, the disclosure also provides methods for detecting a microbial co-infection of a subject suffering from a SARS-CoV2 infection.

公开(公告)号：US20200048713A1

公开(公告)日：2020-02-13

申请号：US16500929

申请日：2018-04-05

申请人： CORNELL UNIVERSITY

发明人： Iwijn DE VLAMINCK ,  John Richard LEE ,  Philip Smith BURNHAM ,  Alexandre Pellan CHENG ,  Manikkam SUTHANTHRIAN ,  Darshana DADHANIA

IPC分类号： C12Q1/6883 ,  C12Q1/689 ,  C12Q1/70 ,  C40B30/06

摘要： The present disclosure is directed to methods of detecting cell-free DNA (cfDNA) in biological samples and using it to quantify organ damage and identify pathogens. In some aspects, the biological samples are from patients who have undergone solid-organ transplantation. The disclosure is also directed to methods of detecting and analyzing methylation patterns in cell-free DNA from organ transplant patients to identify the presence of pathogens as well as quantify contributing tissue proportions as a measurement of the host response.