公开(公告)号：US20190194731A1

公开(公告)日：2019-06-27

申请号：US16327847

申请日：2017-08-23

Applicant: Cancer Research Technology Limited

Inventor： John Burn ,  Mohammed Ghanim Mehdi Alhilal ,  Franscisco Mauro Santibanez-Koref ,  Lisa Redford ,  Michael Stewart Jackson

IPC: C12Q1/6827 ,  C12Q1/6869

CPC classification number: C12Q1/6827 ,  C12Q1/6869 ,  C12Q2535/119

Abstract: The present invention relates to methods and kits for identifying microsatellite instability (MSI) in a sample. In particular it relates to identifying microsatellite instability in a tumor sample, which may be from a subject suspected of having colorectal cancer or Lynch syndrome. The methods and kits can be used to identify mismatch repair defects. More particularly the invention relates to a panel of markers for a sequencing based MSI test, that can differentiate between MSI-H and MSS CRCs. The invention also allows for determination of biological significance, differentiating between PCR and sequencing errors and MSI induced indels/mutations.

公开(公告)号：US11519021B2

公开(公告)日：2022-12-06

申请号：US16327847

申请日：2017-08-23

Applicant: Cancer Research Technology Limited

Inventor： John Burn ,  Mohammed Ghanim Mehdi Alhilal ,  Francisco Mauro Santibanez-Koref ,  Lisa Redford ,  Michael Stewart Jackson

IPC: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present invention relates to methods and kits for identifying microsatellite instability (MSI) in a sample. In particular it relates to identifying microsatellite instability in a tumor sample, which may be from a subject suspected of having colorectal cancer or Lynch syndrome. The methods and kits can be used to identify mismatch repair defects. More particularly the invention relates to a panel of markers for a sequencing based MSI test, that can differentiate between MSI-H and MSS CRCs. The invention also allows for determination of biological significance, differentiating between PCR and sequencing errors and MSI induced indels/mutations.