公开(公告)号：US10590495B2

公开(公告)日：2020-03-17

申请号：US15905330

申请日：2018-02-26

Applicant: Canon U.S.A., Inc.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Brian Bean ,  Scott Corey

IPC: B01L7/00 ,  C12Q3/00 ,  C12Q1/686 ,  B01L3/00

Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.

公开(公告)号：US20200264055A1

公开(公告)日：2020-08-20

申请号：US16820255

申请日：2020-03-16

Applicant: Canon U.S.A., Inc.

Inventor： Fernando DeCastro ,  Renee Howell ,  Sami Kanderian ,  Johnathan S. Coursey ,  Kenton C. Hasson ,  Scott Sundberg

IPC: G01K11/06 ,  G01K15/00 ,  B01L7/00 ,  B01L3/00 ,  C12Q1/6806

Abstract: The present invention relates to the use of one or more amplicons as temperature calibrators. In some embodiments, the calibrators may be used to calibrate the temperature of a microfluidic channel in which amplification and/or melt analysis is performed. In some embodiments, the amplicons may be genomic, ultra conserved elements and/or synthetic. The amplicon(s) may have a known or expected melt temperature(s). The calibrators may be added to primers of study or may follow or lead the primers of study in the channel. The amplicon(s) may be amplified and melted, and the temperature(s) at which the amplicon(s) melted may be determined. The measured temperature(s) may be compared to the known temperature(s) at which the amplicon(s) was expected to melt. The difference(s) between the measured and expected temperatures may be used to calibrate/adjust one or more temperature control elements used to control and/or detect the temperature of the channel.