公开(公告)号：US20110243853A1

公开(公告)日：2011-10-06

申请号：US12446203

申请日：2007-10-24

申请人： Catriona Helen M. Jamieson ,  Ken Kaushansky ,  Charlene Barroga ,  Ifat Geron ,  Annelie Schairer ,  Edward Kavalerchik

发明人： Catriona Helen M. Jamieson ,  Ken Kaushansky ,  Charlene Barroga ,  Ifat Geron ,  Annelie Schairer ,  Edward Kavalerchik

IPC分类号： A01K67/033 ,  A61K49/00 ,  C12Q1/02

CPC分类号： A01K67/0271 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2227/105 ,  A01K2267/0381 ,  C12N9/1205 ,  G01N33/5088

摘要： Non human animal models are provided for diseases involving erythroid function, particularly myeloproliferative disease. The models are useful for testing and screening of biologically active agents that affect erythropoiesis, and erythroid function. In the animal models of the invention, a hematopoietic stem or progenitor cell (HSC) population that has been genetically altered by the introduction of a mutant JAK2 coding sequence is transplanted into an immunocompromised, xenogeneic, non-human recipient. The recipient animal is engrafted with the cell population at a high frequency, and develops a myeloproliferative disorder characterized by polycythemia.

摘要翻译： 非人类动物模型适用于涉及红系功能的疾病，特别是骨髓增生性疾病。 该模型对于影响红细胞生成和红细胞功能的生物活性剂的测试和筛选是有用的。 在本发明的动物模型中，通过引入突变体JAK2编码序列遗传改变的造血干细胞或祖细胞（HSC）群体被移植到免疫受损的异种非人受体中。 受体动物以高频率移植细胞群，并发展出以红细胞增多症为特征的骨髓增生性疾病。