公开(公告)号：US20230348936A1

公开(公告)日：2023-11-02

申请号：US18301818

申请日：2023-04-17

申请人： Cevec Pharmaceuticals, GmbH

发明人： Kerstin Hein ,  Nicole Faust ,  Silke Wissing

IPC分类号： C12N15/86 ,  C12N15/01 ,  C12N15/63

CPC分类号： C12N15/86 ,  C12N15/01 ,  C12N15/63 ,  C12N2750/14152 ,  C12N2750/14122 ,  C12N2750/14143

摘要： The present invention relates to host cells comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68, wherein the internal AAV promoter p19 has been inactivated by one or more mutations that maintain the functionality of said Rep78 and Rep68 proteins. The present invention further relates to respective nucleic acids and vectors comprising the same, as well as respective methods for the production of AAV.

公开(公告)号：US11685932B2

公开(公告)日：2023-06-27

申请号：US16646741

申请日：2018-09-18

申请人： Cevec Pharmaceuticals, GmbH

发明人： Kerstin Hein ,  Nicole Faust ,  Silke Wissing

IPC分类号： C12N15/86 ,  C12N15/01 ,  C12N15/63

CPC分类号： C12N15/86 ,  C12N15/01 ,  C12N15/63 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152

摘要： The present invention relates to host cells comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68, wherein the internal AAV promoter p19 has been inactivated by one or more mutations that maintain the functionality of said Rep78 and Rep68 proteins. The present invention further relates to respective nucleic acids and vectors comprising the same, as well as respective methods for the production of AAV.

公开(公告)号：US12060569B2

公开(公告)日：2024-08-13

申请号：US18301818

申请日：2023-04-17

申请人： Cevec Pharmaceuticals, GmbH

发明人： Kerstin Hein ,  Nicole Faust ,  Silke Wissing

IPC分类号： C12N15/86 ,  C12N15/01 ,  C12N15/63

CPC分类号： C12N15/86 ,  C12N15/01 ,  C12N15/63 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152

摘要： The present invention relates to host cells comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68, wherein the internal AAV promoter p19 has been inactivated by one or more mutations that maintain the functionality of said Rep78 and Rep68 proteins. The present invention further relates to respective nucleic acids and vectors comprising the same, as well as respective methods for the production of AAV.

公开(公告)号：US20200277628A1

公开(公告)日：2020-09-03

申请号：US16646741

申请日：2018-09-18

申请人： Cevec Pharmaceuticals, GmbH

发明人： Kerstin Hein ,  Nicole Faust ,  Silke Wissing

IPC分类号： C12N15/86

摘要： The present invention relates to host cells comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68, wherein the internal AAV promoter p19 has been inactivated by one or more mutations that maintain the functionality of said Rep78 and Rep68 proteins. The present invention further relates to respective nucleic acids and vectors comprising the same, as well as respective methods for the production of AAV.