公开(公告)号：US20090075397A1

公开(公告)日：2009-03-19

申请号：US12283469

申请日：2008-09-12

Applicant: Chun-Hung Lin ,  Chuan-Fa Chang

Inventor： Chun-Hung Lin ,  Chuan-Fa Chang

IPC: G01N33/543

CPC classification number: G01N33/54353

Abstract: This invention provides a donor bead for use in an assay, wherein the bead (a) is coated with a layer of hydrogel having directly or indirectly bound thereto a polyacrylamide-supported sugar or a polyacrylamide-supported glycan, and (b) comprises a photosensitizer which, upon excitation by laser light of a suitable wavelength, converts ambient oxygen to singlet state oxygen. This invention also provides an acceptor bead for use in an assay, wherein the bead (a) is coated with a layer of hydrogel having directly or indirectly bound thereto a polyacrylamide-supported sugar or a polyacrylamide-supported glycan, and (b) comprises a chemiluminescer and a fluorophore, whereby when the bead is contacted with singlet state oxygen, the singlet state oxygen reacts with the chemiluminescer which in turn activates the fluorophore so as to cause the emission of light of a predetermined wavelength. This invention further provides related kits, detection methods and characterization methods.

Abstract translation: 本发明提供用于测定中的供体珠粒，其中珠粒（a）涂覆有直接或间接结合聚丙烯酰胺负载的糖或聚丙烯酰胺负载的聚糖的水凝胶层，（b）包含光敏剂 其在经适当波长的激光激发后将环境氧转化为单态态氧。 本发明还提供用于测定中的受体珠粒，其中珠粒（a）涂覆有直接或间接结合有聚丙烯酰胺负载的糖或聚丙烯酰胺负载的聚糖的水凝胶层，（b）包含 化学发光体和荧光团，由此当珠粒与单态态氧接触时，单线态氧与化学发光体反应，其又激活荧光团，从而引起预定波长的光的发射。 本发明还提供了相关试剂盒，检测方法和表征方法。