公开(公告)号：US20250163437A1

公开(公告)日：2025-05-22

申请号：US18949053

申请日：2024-11-15

Applicant: Clemson University Research Foundation

Inventor： Andrei Alexandrov ,  Rui Che ,  Bhoomi Mirani ,  Monireh Mohammadpanah

IPC: C12N15/66 ,  C12N15/10 ,  C12N15/85

Abstract: This invention is directed to methods and compositions for bacteria-free preparation, assembly and expression of heterologous products in eukaryotic cells, including an in vitro method for joining ends of one or more linear double-stranded nucleic acid molecules to form a circular double-stranded nucleic acid construct for use in transfection, electroporation, nucleofection, and/or other form of delivery of the circular nucleic acid construct into a eukaryotic cell, as well as methods for making and compositions comprising the nucleic acid molecules and nucleic acid constructs for use in the methods disclosed herein.