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公开(公告)号：US20170218321A1
公开(公告)日：2017-08-03
申请号：US15492730
申请日：2017-04-20
Applicant: Corning Incorporated
Inventor： GREGORY ROGER MARTIN , Allison Jean Tanner
Abstract: A cell culture insert for use in culturing cells to promote the formation of spheroids and methods of using these spheroid-promoting cell culture inserts. The cell culture insert includes a porous membrane and one or more sidewalls that are non-adherent to cells and cause the cells in the insert to associate with each other and form spheroids.
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公开(公告)号：US20140227775A1
公开(公告)日：2014-08-14
申请号：US14254280
申请日：2014-04-16
Applicant: CORNING INCORPORATED
Inventor： GREGORY ROGER MARTIN , Allison Jean Tanner
IPC: C12M1/12
CPC classification number: C12M23/08 , C12M23/04 , C12M23/24 , C12M23/34 , C12M23/38 , C12M23/58 , C12M25/02 , C12M25/04 , C12M25/06 , C12M29/04 , C12N5/0602
Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.
Abstract translation: 公开了一种培养细胞的多层细胞培养装置。 细胞培养装置被定义为具有与气管空间组合的多个细胞培养室的整体结构。 该装置的主体在其中赋予气体可渗透的膜与气管空间的组合，其将允许细胞培养室与外部环境之间的气体自由流动。 烧瓶主体还包括孔，其将允许通过针或插管进入细胞生长室。 设备的尺寸和可选的颈部和帽部分的位置允许其通过标准自动化测定设备进行操作，进一步使得该设备对于高通量应用是理想的。
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公开(公告)号：US20140065709A1
公开(公告)日：2014-03-06
申请号：US14075446
申请日：2013-11-08
Applicant: Corning Incorporated
Inventor： GREGORY ROGER MARTIN , Allison Jean Tanner
IPC: C12M1/04
CPC classification number: C12M23/08 , C12M23/04 , C12M23/24 , C12M23/34 , C12M23/38 , C12M23/58 , C12M25/02 , C12M25/04 , C12M25/06 , C12M29/04 , C12N5/0602
Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.
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