公开(公告)号：US20110214200A1

公开(公告)日：2011-09-01

申请号：US12904450

申请日：2010-10-14

申请人： DAOLONG WANG ,  LIBARDO ANDRES GUTIERREZ ROJAS ,  XI CHEN ,  JOSEPH CLARKE ,  MICHAEL G. MUSZYNSKI ,  PAUL ALTENDORF ,  SARAH ALISSA FORRESTER ,  LYNN SENIOR ,  VENKATA KRISHNA KISHORE

发明人： DAOLONG WANG ,  LIBARDO ANDRES GUTIERREZ ROJAS ,  XI CHEN ,  JOSEPH CLARKE ,  MICHAEL G. MUSZYNSKI ,  PAUL ALTENDORF ,  SARAH ALISSA FORRESTER ,  LYNN SENIOR ,  VENKATA KRISHNA KISHORE

IPC分类号： A01H1/02 ,  C12Q1/68 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10 ,  C07H21/04

CPC分类号： C12N15/8261 ,  A01H1/02 ,  A01H1/04 ,  A01H5/10 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

摘要： Methods for introgressing an allele of interest of a locus associated with a yield trait into Zea mays germplasm are provided. In some embodiments, the methods include providing a Zea mays plant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by PCR amplification of a Zea mays nucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest into Zea mays germplasm that lacks the allele. Also provided are methods for identifying Zea mays plants that contain at least one allele associated with improved yield, improved maize plants, elite Zea mays plants, biomass produced from improved Zea mays plants, isolated and purified genetic markers, and compositions that include an amplification primer pair capable of amplifying a Zea mays nucleic acid to generate a Zea mays marker amplicon.

摘要翻译： 提供了将与产量性状相关的基因座感兴趣等位基因浸入玉米种质的方法。 在一些实施方案中，所述方法包括提供包含与产量性状相关的基因座的目的等位基因的玉米种植体，其中与产量性状相关的基因座可通过具有一对玉米的玉米核酸的PCR扩增来鉴定 寡核苷酸引物，并将感兴趣的等位基因渗入缺少等位基因的玉米种质。 还提供了用于鉴定含有至少一个与改良产量相关的等位基因的Zea mays植物的方法，改良的玉米植物，精英玉米植物，由改良的Zea mays植物产生的生物，分离和纯化的遗传标记，以及包含扩增引物 能够扩增Zea mays核酸以产生Zea mays标记扩增子。