公开(公告)号：US12077801B2

公开(公告)日：2024-09-03

申请号：US16500497

申请日：2018-04-06

Applicant: DSM IP Assets B.V.

Inventor： Werner Bonrath ,  Martin Schürmann ,  Michele Tavanti ,  Fabio Parmeggiani ,  Nicholas Turner ,  Andrea Mattevi ,  Jose Ruben Casstellanos

IPC: C12P7/26 ,  C12N9/02

CPC classification number: C12P7/26 ,  C12N9/0081 ,  C12Y114/15006

Abstract: The present invention relates to novel process for the production of ketoisophorone via biocatalytic conversion of isophorone, in particular a one-pot biocatalytic system for conversion of α-isophorone in a two-step oxidation process, with a first oxidation being catalyzed by a heme containing oxidoreductase such as a cytochrome P450 monooxygenase followed by another oxidation which can be either a chemical reaction or a biocatalytic reaction, in particular wherein the oxidation is catalyzed by an NAD(P) or NADP(H)-dependent oxidoreductase. The invention further provides polypeptides and nucleic acid sequences coding for cytochrome P450 monooxygenase with modified (higher) substrate selectivity, total turnover numbers and/or (re)activity compared to the wild-type enzyme. Ketoisophorone is useful as building block in the synthesis of vitamins and carotenoids.