公开(公告)号：US5939601A

公开(公告)日：1999-08-17

申请号：US722626

申请日：1996-09-27

申请人： Daniel F. Klessig ,  Yinong Yang

发明人： Daniel F. Klessig ,  Yinong Yang

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H4/00 ,  A01H5/10

CPC分类号： C12N15/8283 ,  C07K14/415 ,  C12N15/8282

摘要： An isolated nucleic acid molecule is provided which encodes a tobacco myb homologue involved in the regulation of disease resistance in plants. The encoded protein comprises a basic N-terminal region with two imperfect tryptophan repeats of 53 and 51 amino acids, a potential ATP/GTP binding site or P-loop, a redox sensitive cysteine and a nuclear localization sequence. The acidic C terminus of Myb1 forms amphipathic .alpha. helices which are characteristic of transcriptional activation domains. The invention also provides novel Myb1 protein and antibodies thereto. Additionally, the invention provides novel transgenic plants with enhanced disease resistance to certain pathogens.

摘要翻译： 提供分离的核酸分子，其编码参与植物中抗病性调节的烟草myb同源物。 编码的蛋白质包含具有53和51个氨基酸的两个不完全色氨酸重复的碱性N末端区域，潜在的ATP / GTP结合位点或P环，氧化还原敏感半胱氨酸和核定位序列。 Myb1的酸性C末端形成转录激活结构域特征的两亲性α螺旋。 本发明还提供了新颖的Myb1蛋白及其抗体。 另外，本发明提供对某些病原体具有增强的抗病性的新型转基因植物。

公开(公告)号：US07345219B2

公开(公告)日：2008-03-18

申请号：US10768886

申请日：2004-01-31

申请人： Yinong Yang ,  Lizhong Xiong

发明人： Yinong Yang ,  Lizhong Xiong

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87

CPC分类号： C12N9/1205 ,  C07H21/04 ,  C12N15/8271

摘要： The present invention relates to the mitogen-activated protein kinase called MAPK5. The rice MAPK5 gene, its protein and kinase activity were induced by abscisic acid, pathogen infection, wounding, drought, salt and cold temperature. However, suppression of MAPK5 expression and kinase activity in dsRNAi transgenic plants resulted in constitutive expression of pathogenesis-related genes such as PR-1 and PR-10 but enhanced resistance to fungal and bacterial pathogens. In contrast, overexpressed transgenic lines exhibited elevated MAPK5 kinase activity and increased tolerance to drought, salt and cold stresses. This invention provides methods for increasing tolerance to abiotic and biotic stress in plant using MAPK5.

摘要翻译： 本发明涉及称为MAPK5的丝裂原活化蛋白激酶。 水稻MAPK5基因，其蛋白和激酶活性由脱落酸，病原体感染，伤口，干旱，盐和冷温度诱导。 然而，dsRNAi转基因植物中MAPK5表达和激酶活性的抑制导致发病相关基因如PR-1和PR-10的组成型表达，但对真菌和细菌病原体的抗性增强。 相比之下，过表达的转基因品系表现出升高的MAPK5激酶活性和增加的对干旱，盐和冷应激的耐受性。 本发明提供了使用MAPK5增加植物中非生物和生物胁迫耐受性的方法。