公开(公告)号：US20220025441A1

公开(公告)日：2022-01-27

申请号：US17328762

申请日：2021-05-24

Applicant: Delta Electronics, Inc.

Inventor： Yi-Chen Li ,  Chih-Cheng Tsou ,  Min-Hsien Wu ,  Hsin-Yao Wang ,  Chien-Ru Lin

IPC: C12Q1/689 ,  C12Q1/686

Abstract: A method and a kit for detecting Mycobacterium tuberculosis are provided. The method includes a step of performing a nested qPCR assay to a specimen. The nested qPCR assay includes a first round of amplification using external primers and a second round of amplification using internal primers and a probe. The external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.

公开(公告)号：US11965217B2

公开(公告)日：2024-04-23

申请号：US17328762

申请日：2021-05-24

Applicant: Delta Electronics, Inc.

Inventor： Yi-Chen Li ,  Chih-Cheng Tsou ,  Min-Hsien Wu ,  Hsin-Yao Wang ,  Chien-Ru Lin

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/689

CPC classification number: C12Q1/689 ,  C12Q1/686

Abstract: A method and a kit for detecting Mycobacterium tuberculosis are provided. The method includes a step of performing a nested qPCR assay to a specimen. The nested qPCR assay includes a first round of amplification using external primers and a second round of amplification using internal primers and a probe. The external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.

公开(公告)号：US11174520B2

公开(公告)日：2021-11-16

申请号：US16287585

申请日：2019-02-27

Applicant: DELTA ELECTRONICS, INC.

Inventor： Chih-Cheng Tsou ,  Min-Hsien Wu ,  Wen-Pin Chou ,  Hsin-Yao Wang ,  Chien-Ru Lin

IPC: C12Q1/689 ,  C12Q1/6883

Abstract: A method and a kit for detecting mycobacterium are provided. The method includes steps of: providing a sample; providing a pair of primers, which is selected from a group consisting of a sequence having about 45% to 100% similar to SEQ ID NO. 1, a sequence having about 60% to 100% similar to SEQ ID NO. 2, a sequence complementary thereof; performing a polymerase chain reaction by using the set of primers and the sample to obtain a product; and analyzing the product to detect the presence of the mycobacterium.

公开(公告)号：US20190264261A1

公开(公告)日：2019-08-29

申请号：US16287585

申请日：2019-02-27

Applicant: DELTA ELECTRONICS, INC.

Inventor： Chih-Cheng Tsou ,  Min-Hsien Wu ,  Wen-Pin Chou ,  Hsin-Yao Wang ,  Chien-Ru Lin

IPC: C12Q1/689

Abstract: A method and a kit for detecting mycobacterium are provided. The method includes steps of: providing a sample; providing a pair of primers, which is selected from a group consisting of a sequence having about 45% to 100% similar to SEQ ID NO. 1, a sequence having about 60% to 100% similar to SEQ ID NO. 2, a sequence complementary thereof; performing a polymerase chain reaction by using the set of primers and the sample to obtain a product; and analyzing the product to detect the presence of the mycobacterium.