公开(公告)号：US20130123744A1

公开(公告)日：2013-05-16

申请号：US13138722

申请日：2010-03-26

申请人： Derin C. Walters ,  Alan D. King ,  Anna-Karin Maltais (formerly Anna-Karin Roos) ,  Britta Wahren ,  Kristian Haller (formerly Kristian Hallermalm) ,  Andreas Brave ,  Robert J. Walters

发明人： Richard E. Walters ,  Derin C. Walters ,  Alan D. King ,  Anna-Karin Maltais (formerly Anna-Karin Roos) ,  Britta Wahren ,  Kristian Haller (formerly Kristian Hallermalm) ,  Andreas Brave

IPC分类号： A61N1/30

CPC分类号： A61N1/306 ,  A61K39/00 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/54 ,  A61N1/327 ,  C12N2770/24134

摘要： The invention provides a method and apparatus for the delivery of polynucleotide cancer vaccines to increase T cell response and reduce pain due to long electric waveform application and muscle contractions. The method includes the steps of: (a.) administering a polynucleotide cancer vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity of the administration site, and (c.) applying a sequence of at least three electrical waveforms to deliver the polynucleotide cancer vaccine into skin cells by electroporation. The sequence has at least one of the following characteristics {1} at least two of the waveforms differ in amplitude, {2} at least two of the waveforms differ in width, and (3) a first waveform interval for a first set of two of the waveforms is different from a second waveform interval for a second set of two of the waveforms.

摘要翻译： 本发明提供了用于递送多核苷酸癌症疫苗以增加T细胞应答并减少由于长的电波形应用和肌肉收缩引起的疼痛的方法和装置。 该方法包括以下步骤：（a）在给药部位向皮肤施用多核苷酸癌症疫苗，（b）在给药部位附近向皮肤施用针电极，（c） 至少三个电波形的序列，以通过电穿孔将多核苷酸癌症疫苗递送到皮肤细胞中。 该序列具有以下特征中的至少一个{1}，至少两个波形幅度不同，{2}至少两个波形的宽度不同，（3）第一组二分之一的第一波形间隔 的波形与第二组波形的第二波形间隔不同。