公开(公告)号：US20190194686A1

公开(公告)日：2019-06-27

申请号：US16147741

申请日：2018-09-29

Applicant: Development Center for Biotechnology

Inventor： HSIN-LIN LU ,  CHIEN-I LIN ,  CHAO-YI TENG

IPC: C12N15/85 ,  C12N15/113

CPC classification number: C12N15/85 ,  C12N15/113 ,  C12N2015/8518

Abstract: A host cell for protein expression having a lower expression level of a gene, as compared to a wild-type cell, wherein the gene is selected from HDAC8, Dab2, Caspase3, Sys1, Ergic3, Grasp, Trim 23, or a combination thereof. The host cells are CHO cells. The lower expression level of the gene results from RNA interference, which may be achieved by transfecting a vector that contains an shRNA targeting the gene.