公开(公告)号：US20180371088A1

公开(公告)日：2018-12-27

申请号：US16016414

申请日：2018-06-22

Applicant: Development Center for Biotechnology

Inventor： Chia-Cheng WU ,  Tzu-Yin LIN ,  Chao-Yang HUANG ,  Yu-Jung CHEN ,  Jei-Hwa YU ,  Chen-Li CHIEN

IPC: C07K16/28 ,  C07K16/30 ,  A61P35/00

Abstract: An asymmetric heterodimeric antibody includes a knob structure formed in a CH3 domain of a first heavy chain; a hole structure formed in a CH3 domain of a second heavy chain, wherein the hole structure is configured to accommodate the knob structure so that a heterodimeric antibody is formed; and a T-cell targeting domain fused to the CH3 domain of the first heavy chain or the second heavy chain, wherein the T-cell targeting domain binds specifically to an antigen on the T-cell. The T-cell targeting domain is a ScFv or Fab derived from an anti-CD3 antibody. The asymmetric heterodimeric antibody may have L234A and L235A mutations or L235A and G237A such that its effector binding is compromised.

公开(公告)号：US20180371089A1

公开(公告)日：2018-12-27

申请号：US16016525

申请日：2018-06-22

Applicant: Development Center for Biotechnology

Inventor： Chia-Cheng WU ,  Tzu-Yin LIN ,  Yu-Jung CHEN ,  Chao-Yang HUANG

IPC: C07K16/28

Abstract: A bispecific anti-Globo H antibody includes an anti-Globo H antibody that binds specifically to Globo H; and a T-cell targeting domain fused to a CH3 domain of a heavy chain of the anti-Globo H antibody, wherein the T-cell targeting domain binds specifically to an antigen on T-cells; and wherein the anti-Globo H antibody comprises mutations at an effector binding site such that the bispecific anti-Globo H antibody has a diminished effector function. The T-cell targeting domain is a ScFv or Fab from an anti-CD3 antibody.