公开(公告)号：US5420244A

公开(公告)日：1995-05-30

申请号：US103742

申请日：1993-08-06

申请人： Donna L. Rudolph ,  Renu B. Lal

发明人： Donna L. Rudolph ,  Renu B. Lal

IPC分类号： C07K14/16 ,  A61K38/00

CPC分类号： C07K14/005 ,  C12N2740/16322

摘要： The invention provides antigenic peptides derived from immunodominant epitopes of the HTLV-I tax or rex proteins that are immunoreactive with antibodies associated with disease in HTLV-I infected subjects. More specifically, the invention provides antigenic peptides consisting essentially of the amino acid sequences defined in the sequence listing by SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7 and 8 and antigenic fragments thereof. The invention provides a method of diagnosing HTLV-I associated myelopathy (HAM) or a predisposition thereto, comprising the steps of: (a) contacting an antibody containing sample from the subject with a detectable amount of a peptide of the invention or an antigenic fragment thereof; and (b) detecting the reaction of the peptide with an antibody in the sample, the reaction indicating HTLV-I associated myelopathy or a predisposition thereto. The invention also provides a method of diagnosing adult T-cell leukemia or a predisposition thereto in a subject, comprising the steps of: (a) contacting an antibody containing sample from the subject with a detectable amount of the peptide of SEQ ID NO: 1; and (b) detecting the reaction of the peptide with an antibody in the sample, the reaction indicating adult T-cell leukemia or predisposition thereto.

摘要翻译： 本发明提供了衍生自HTLV-1的免疫显性表位的抗原肽，或与HTLV-1感染的受试者中与疾病相关的抗体免疫反应的rex蛋白。 更具体地，本发明提供了基本上由SEQ ID NO：1,2,3,4,5,6,7和8的序列表中定义的氨基酸序列及其抗原性片段组成的抗原肽。 本发明提供了诊断HTLV-1相关性脊髓病（HAM）或其倾向的方法，其包括以下步骤：（a）使来自受试者的抗体含有可检测量的本发明的肽或抗原性片段 的; 和（b）检测样品中肽与抗体的反应，表明HTLV-1相关的脊髓病或其倾向的反应。 本发明还提供了一种在受试者中诊断成人T细胞白血病或倾向于其的方法，包括以下步骤：（a）使来自受试者的含抗体的样品与可检测量的SEQ ID NO：1的肽接触 ; 和（b）检测样品中肽与抗体的反应，该反应指示成年T细胞白血病或其倾向。