公开(公告)号：US20170298451A1

公开(公告)日：2017-10-19

申请号：US15495937

申请日：2017-04-24

Applicant: Dow AgroSciences LLC

Inventor： Lasantha Chandana Ubayasena ,  Zoe C. Ehlert ,  Manju Gupta ,  Chandra - Shekara A. Channabasavaradhya

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/156

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.