公开(公告)号：US20130102001A1

公开(公告)日：2013-04-25

申请号：US13656251

申请日：2012-10-19

Applicant: Dow AgroSciences LLC

Inventor： Lasantha Chandana Ubayasena ,  Zoe Ehlert ,  Chandra Shekara A. Channabasavaradhya

IPC: G01N21/64

CPC classification number: C12Q1/6895 ,  C12Q1/6851 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-2 gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

Abstract translation: 本发明部分涉及终点TaqMan PCR分析用于油菜中fad-2基因的检测和高通量接合度分析。 本发明进一步涉及使用野生型DNA作为用于确定接合性的参考。 这些和其它相关方法可用于唯一地鉴定包含受试基因的卡诺拉系的接合度和品种。 本发明还提供了用于确定来自卡诺拉植物或种子的样品的接合性的相关试剂盒。

公开(公告)号：US09663832B2

公开(公告)日：2017-05-30

申请号：US13656270

申请日：2012-10-19

Applicant: Dow AgroSciences LLC

Inventor： Lasantha Chandana Ubayasena ,  Zoe Ehlert ,  Manju Gupta ,  Chandra Shekara A. Channabasavaradhya

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/156

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

公开(公告)号：US20130102002A1

公开(公告)日：2013-04-25

申请号：US13656270

申请日：2012-10-19

Applicant: Dow AgroSciences LLC

Inventor： Lasantha Chandana Ubayasena ,  Zoe Ehlert ,  Manju Gupta ,  Chandra Shekara A. Channabasavaradhya

IPC: G01N21/64

CPC classification number: C12Q1/6895 ,  C12Q2600/156

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

Abstract translation: 本发明的一部分涉及终点TaqMan PCR分析用于卡诺拉油菜中fad-3c基因的检测和高通量接合度分析。 本发明进一步涉及使用野生型DNA作为用于确定接合性的参考。 这些和其它相关方法可用于唯一地鉴定包含受试基因的卡诺拉系的接合度和品种。 本发明还提供了用于确定来自卡诺拉植物或种子的样品的接合性的相关试剂盒。