公开(公告)号：US20150191721A1

公开(公告)日：2015-07-09

申请号：US14409416

申请日：2013-06-13

Applicant: Dow AgroSciences LLC

Inventor： Matthew Kelker ,  Aaron T. Woosley

IPC: C12N15/11 ,  C12P21/00 ,  C12N15/64

CPC classification number: C12N15/11 ,  C12N15/63 ,  C12N15/64 ,  C12N15/78 ,  C12N2310/353 ,  C12P21/00

Abstract: Compositions and methods for improving expression of a recombinant protein or polypeptide of interest in a host cell are provided. Compositions comprising a polynucleotide coding sequence for a BTBooster are provided using a plant optimized system. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a recombinant protein or polypeptide of interest in a host cell. Methods comprising the codon optimization of a polynucleotide coding sequence for increased expression in a bacterial host are provided. The codon optimization method can be used in designing a polynucleotide coding sequence which expresses robust levels of protein in a bacterial host cell.

Abstract translation: 提供了用于改善宿主细胞中目的重组蛋白或多肽表达的组合物和方法。 使用植物优化系统提供包含BTBooster的多核苷酸编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达目标重组蛋白或多肽。 提供了包括用于在细菌宿主中增加表达的多核苷酸编码序列的密码子优化的方法。 密码子优化方法可用于设计在细菌宿主细胞中表达强大的蛋白质水平的多核苷酸编码序列。

公开(公告)号：US09534218B2

公开(公告)日：2017-01-03

申请号：US14409416

申请日：2013-06-13

Applicant: Dow AgroSciences LLC

Inventor： Matthew Kelker ,  Aaron T. Woosley

IPC: C12N15/64 ,  C12N15/11 ,  C12N15/63 ,  C12N15/78 ,  C12P21/00 ,  C12N1/21 ,  C12N15/67

CPC classification number: C12N15/11 ,  C12N15/63 ,  C12N15/64 ,  C12N15/78 ,  C12N2310/353 ,  C12P21/00

Abstract: Compositions and methods for improving expression of a recombinant protein or polypeptide of interest in a host cell are provided. Compositions comprising a polynucleotide coding sequence for a BTBooster are provided using a plant optimized system. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a recombinant protein or polypeptide of interest in a host cell. Methods comprising the codon optimization of a polynucleotide coding sequence for increased expression in a bacterial host are provided. The codon optimization method can be used in designing a polynucleotide coding sequence which expresses robust levels of protein in a bacterial host cell.

Abstract translation: 提供了用于改善宿主细胞中目的重组蛋白或多肽表达的组合物和方法。 使用植物优化系统提供包含BTBooster的多核苷酸编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达目标重组蛋白或多肽。 提供了包括用于在细菌宿主中增加表达的多核苷酸编码序列的密码子优化的方法。 密码子优化方法可用于设计在细菌宿主细胞中表达强大的蛋白质水平的多核苷酸编码序列。