公开(公告)号：US20200224269A1

公开(公告)日：2020-07-16

申请号：US16819972

申请日：2020-03-16

Applicant: Dow AgroSciences LLC

Inventor： PoHao Wang ,  Shreedharan Sriram ,  Lakshmi Sastry-Dent

IPC: C12Q1/6876 ,  G16B25/00 ,  C12Q1/6895 ,  C12N15/82 ,  G16B20/00

Abstract: The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold value for predicting gene silencing. The methods and/or systems provided herein can be used in high-throughput setting for screening large number of transformed event in a relatively short period of time as compared to existing technologies.