公开(公告)号：US09920331B2

公开(公告)日：2018-03-20

申请号：US14586135

申请日：2014-12-30

Applicant: Dow AgroSciences LLC

Inventor： Ruihua Ren ,  Bruce A. Nagel ,  Liang Ye ,  Yanxin Star Gao ,  Ryan Gibson ,  Sushmitha Paulraj ,  Tyler Mansfield ,  Jafar Mammadov ,  Siva P. Kumpatla ,  Steven A. Thompson

IPC: C12N15/82 ,  A01H1/02 ,  C07K14/415

CPC classification number: C12N15/8287 ,  A01H1/02 ,  C07K14/415 ,  C12N15/8289

Abstract: A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

公开(公告)号：US09883643B2

公开(公告)日：2018-02-06

申请号：US14586143

申请日：2014-12-30

Applicant: Dow AgroSciences LLC

Inventor： Ruihua Ren ,  Bruce A. Nagel ,  Ryan Gibson ,  Yanxin Star Gao ,  Jafar Mammadov

IPC: A01H1/02 ,  A01H1/04 ,  C12Q1/68 ,  C07K14/415

CPC classification number: A01H1/04 ,  A01H1/02 ,  C07K14/415 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The present disclosure provides a method for selecting a plant comprising a functional restorer gene for maize S-type cytoplasmic male sterility comprising the steps of (a) screening a population of plants for at least one marker nucleic acid, wherein the marker nucleic acid comprises an allele linked to the functional restorer gene for maize S-type cytoplasmic male sterility; (b) detecting the marker nucleic acid; (c) identifying a plant comprising the marker nucleic acid; and (d) selecting the plant comprising the marker nucleic acid, wherein the plant comprising the marker nucleic acid further comprises the functional restorer gene for maize S-type cytoplasmic male sterility. The present disclosure also provides methods for restoring fertility in a progeny of an S-type cytoplasmic male sterile plant and methods for transferring an Rf3 gene into a progeny plant.