公开(公告)号：US20160313317A1

公开(公告)日：2016-10-27

申请号：US15026070

申请日：2013-09-30

Applicant: EMPIRE TECHNOLOGY DEVELOPMENT LLC

Inventor： Mattias Lennart ASLUND

IPC: G01N33/543 ,  G01N21/64

CPC classification number: G01N33/54373 ,  B82Y30/00 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54346 ,  G01N33/54366 ,  G01N33/54393

Abstract: Methods, systems, and kits are disclosed for determining the presence of a biomolecule. An assay plate may be composed of nanostructures capable of binding the biomolecule. Reporter species may be associated with the nanostructure-bound biomolecules. The assay plate may be illuminated with radiation capable of inducing plasmonic fields near the nanostructures. The fields may cause nearby reporter species to fluoresce. Nanostructures may be fabricated by illuminating nanoparticles in the presence of linker species. The induced plasmonic fields may cause the linker species to bind to the nanoparticles at field hotspots. Binder species conjugated with the linker species may form capture species capable of binding the biomolecule. A system for measuring the presence of a biomolecules may include a chamber that may be light tight, a source of illumination, and a photodetector. A kit for such a system may include an assay plate and solutions for the assay.

Abstract translation: 公开了用于确定生物分子存在的方法，系统和试剂盒。 测定板可以由能够结合生物分子的纳米结构组成。 记录物种可能与纳米结构结合的生物分子相关。 可以用能够诱导纳米结构附近的等离子体场的辐射照射测定板。 这些领域可能导致附近的报道物种发荧光。 纳米结构可以通过在接头物质的存在下照射纳米颗粒来制造。 诱导的等离子体场可能导致接头物质在场热点处与纳米颗粒结合。 与接头物质结合的粘结剂物质可以形成能够结合生物分子的捕获物质。 用于测量生物分子的存在的系统可以包括可以是不透光的室，照明源和光电检测器。 用于这种系统的试剂盒可以包括测定板和用于测定的溶液。