公开(公告)号：US20150197762A1

公开(公告)日：2015-07-16

申请号：US14391066

申请日：2013-04-09

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA

Inventor： Juliana Dantas Dealmeida ,  Leila Maria Gomes Barros ,  Mauro Carneiro ,  Alan Carvalho Andrade ,  Felipe Rodrigues Da Silva ,  Luiz Filipe Protasio Pereira ,  Michelle Guitton Cotta ,  Mirian Therezinha Souza Da Eira

IPC: C12N15/82

CPC classification number: C12N15/8234

Abstract: The present invention relates to a promoter sequence specific for expression of genes of interest in fruit endosperm. The invention further describes DNA constructs that contain the promoter, as well as the method using such constructs to produce transgenic plants, plant cells or protoplasts. The expression of the transgene only in the organ of interest enables accumulation of the exogenous transcript only in the fruit and constitutes an advantage over the constitutive expression, since it favors implementation of strategies that aim at obtaining cultivars having higher aggregate value like: adaptation to environmental stress, tolerance to pathogens, pests and agricultural defensives, increase in the nutritional and therapeutic value and a new alternative to systems of expression in plant organism, generating more competitive cultivars at lower costs. Besides, the present invention also presents a sequence with constitutive expression, providing an alternative to the implementation of expression system where the constitutive expression is required. The invention has the objective of increasing the economic, social and, environmental and biosafety benefits associated to the genetic transformation.

Abstract translation: 本发明涉及对果实胚乳中感兴趣的基因表达特异性的启动子序列。 本发明还描述了含有启动子的DNA构建体，以及使用这种构建体产生转基因植物，植物细胞或原生质体的方法。 仅在感兴趣的器官中转基因的表达使得外源转录物仅能够在果实中积累，并且构成了优于组成型表达的优点，因为它有利于实现旨在获得具有较高总体值的品种的策略，如：适应环境 胁迫，对病原体的耐受性，害虫和农业防御力，营养和治疗价值的增加，以及植物生物体内表达系统的新选择，以更低的成本产生更具竞争力的品种。 此外，本发明还提供了具有组成型表达的序列，为实现需要组成型表达的表达系统提供了替代方案。 本发明的目的是增加与遗传转化相关的经济，社会和环境以及生物安全方面的利益。

公开(公告)号：US10988771B2

公开(公告)日：2021-04-27

申请号：US14391066

申请日：2013-04-09

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA—EMBRAPA

Inventor： Juliana Dantas De Almeida ,  Leila Maria Gomes Barros ,  Mauro Carneiro ,  Alan Carvalho Andrade ,  Felipe Rodrigues Da Silva ,  Luiz Filipe Protasio Pereira ,  Michelle Guitton Cotta ,  Mirian Therezinha Souza Da Eira

IPC: C12N15/82

Abstract: The present disclosure relates to a promoter sequence from the lipid transfer protein 1 gene of Coffea arabica. The present disclosure further describes DNA constructs that contain the promoter, as well as methods of producing transgenic plants, plant cells or protoplasts, using such constructs.