公开(公告)号：US20210065362A1

公开(公告)日：2021-03-04

申请号：US16644392

申请日：2018-10-30

Applicant: ESSEN INSTRUMENTS, INC. D/B/A ESSEN BIOSCIENCE, INC.

Inventor： Daniel APPLEDORN ,  Eric ENDSLEY ,  Nevine HOLTZ ,  Brad NEAGLE ,  David ROCK ,  Kirk SCHROEDER

IPC: G06T7/00 ,  G06T7/254 ,  G16B45/00 ,  G06K9/00

Abstract: Systems and methods are provided for automatically imaging and analyzing cell samples in an incubator. An actuated microscope operates to generate images of samples within wells of a sample container across days, weeks, or months. A plurality of images is generated for each scan of a particular well, and the images within such a scan are used to image and analysis metabolically active cells in the well. Tins analysis includes generating a “range image” by subtracting the minimum intensity value, across the scan, for each pixel from the maximum intensity value. This range image thus emphasizes cells or portions of cells that exhibit changes in activity over a scan period (e.g., neurons, myocytes, cardiomyocytes) while de-emphasizing regions that exhibit consistently high intensities when images (e.g., regions exhibiting a great deal of autofluorescence unrelated to cell activity).