公开(公告)号：US20210308335A1

公开(公告)日：2021-10-07

申请号：US14047948

申请日：2013-10-07

Applicant: EWHA UNIVERSITY -INDUSTRY COLLABORATION FOUNDATION

Inventor： Seung Jin Lee ,  Sol Han ,  In Kyong Shim ,  Young Il Yang ,  Yang Soo Jang ,  Mi Ra Jung ,  Hye Jin Chung

IPC: A61L27/56 ,  D01D5/00 ,  D01D7/00 ,  D01D11/00 ,  D01F6/62 ,  A61L27/36 ,  C12M1/12

Abstract: The present invention relates to a fibrous 3-dimensional porous scaffold obtained by electro-spinning for tissue regeneration and a method for preparing the same.

公开(公告)号：US20130295060A1

公开(公告)日：2013-11-07

申请号：US13886663

申请日：2013-05-03

Applicant: Inje University Industry-Academic Cooperation Foundation ,  Ewha University-Industry Collaboration Foundation

Inventor： Young Il YANG ,  Seung Jin Lee ,  Hyeong In Kim ,  Jong Tae Kim ,  Soon Ho Cheong

IPC: A61K35/34

CPC classification number: A61K35/34 ,  C12N5/0657 ,  C12N5/0692 ,  C12N2501/999 ,  C12N2533/52

Abstract: Disclosed is a method for culturing myocardium-resident cardiac progenitor cells, comprising: embedding myocardial fragments into hydrogel; culturing the myocardial fragment into hydrogel; degrading only the hydrogel to recover cardiac progenitor cells grown out of the myocardial fragment to the hydrogel; and amplifying the cardiac progenitor cells in vitro. Also, the cardiac progenitor cells, a method for differentiating the same, and the use thereof as cell therapeutic agent for heart diseases are provided. In addition to possessing the potential to differentiate into cardiomyocytes, osteoblasts, adipocytes, chondrocytes, vascular endothelial cells, smooth muscle cells, neural cells, and skeletal muscle cells, the myocardium-resident cardiac progenitor cells can spontaneously differentiate into cardiomyocytes even in the absence of a special differentiation inducing agent. Thus, the cardiac progenitor cells can be used to produce bio-active medicines such as cell therapeutics and tissue engineering therapeutics with high industrial applicability.

Abstract translation: 公开了一种培养心肌驻留心脏祖细胞的方法，包括：将心肌片段包埋在水凝胶中; 将心肌片段培养成水凝胶; 仅降解水凝胶以将从心肌片段生长的心脏祖细胞回收至水凝胶; 并在体外扩增心脏祖细胞。 此外，提供了心脏祖细胞，其分化方法及其作为心脏病的细胞治疗剂的用途。 除了具有分化成心肌细胞，成骨细胞，脂肪细胞，软骨细胞，血管内皮细胞，平滑肌细胞，神经细胞和骨骼肌细胞的潜力之外，心肌驻留的心脏祖细胞也可以自发分化成心肌细胞，即使没有 一种特殊的分化诱导剂。 因此，心脏祖细胞可用于生产具有高工业应用性的生物活性药物，例如细胞治疗剂和组织工程治疗剂。