Extraction of water soluble biomaterials from fluids using a carbon dioxide/surfactant mixture
    2.
    发明授权
    Extraction of water soluble biomaterials from fluids using a carbon dioxide/surfactant mixture 失效
    使用二氧化碳/表面活性剂混合物从流体中提取水溶性生物材料

    公开(公告)号:US06562605B1

    公开(公告)日:2003-05-13

    申请号:US08747474

    申请日:1996-11-12

    IPC分类号: C07K100

    摘要: A method is provided for the extraction of water soluble biomaterials such as enzymes or proteins into carbon dioxide utilizing certain carbon dioxide soluble surfactants. The extraction can be performed on an aqueous solution, a fermentation broth or a fluid. The method includes the process steps of forming a carbon dioxide/surfactant mixture which involves dissolving carbon dioxide soluble surfactant(s) in carbon dioxide. The carbon dioxide can be in a liquid or supercritical form and the surfactant includes tail and head groups that interact with the biomaterials. Further, the mixture is added to the aqueous solution, fermentation broth or liquid under conditions to allow for extraction of the biomaterials. The method further includes depressurizing and/or temperature adjusting to remove the water soluble biomaterials. The surfactants include fluroethers, oligomers of propylene-oxide, siloxanes, etc. The biomaterials include proteins or enzymes. The carbon dioxide is suberitical or supercritical.

    摘要翻译: 提供了一种利用某些二氧化碳可溶性表面活性剂将水溶性生物材料如酶或蛋白质提取到二氧化碳中的方法。 提取可以在水溶液,发酵液或流体上进行。 该方法包括形成二氧化碳/表面活性剂混合物的方法步骤,其包括将二氧化碳可溶性表面活性剂溶解在二氧化碳中。 二氧化碳可以是液体或超临界形式,并且表面活性剂包括与生物材料相互作用的尾部和头部基团。 此外,在允许提取生物材料的条件下将混合物加入到水溶液,发酵液或液体中。 该方法还包括减压和/或温度调节以除去水溶性生物材料。 表面活性剂包括氟醚,环氧丙烷的低聚物,硅氧烷等。生物材料包括蛋白质或酶。 二氧化碳是非常重要的或超临界的。