METHOD FOR DIVIDING PRIMER PAIRS INTO REACTION CONTAINERS, METHOD FOR AMPLIFYING TARGET NUCLEIC ACIDS, TUBE SET, LIST OF PRIMER PAIRS, AND PROGRAM FOR DIVIDING PRIMER PAIRS INTO REACTION CONTAINERS

    公开(公告)号:US20230220375A1

    公开(公告)日:2023-07-13

    申请号:US18174225

    申请日:2023-02-24

    CPC classification number: C12N15/1048 C12N15/1072 B01L3/5085

    Abstract: Provided is a design method for dividing primer pairs into reaction containers, the design method showing an optimum division example. The design method for dividing primer pairs into reaction containers has a design step of, for a plurality of target nucleic acids, designing a plurality of primer pairs each composed of two types of primers, an evaluation step of evaluating non-specific amplification inducibility between the primer pairs, and an assignment step of performing an assignment to the reaction containers, based on the non-specific amplification inducibility, such that primer pairs having the non-specific amplification inducibility are not present in the same reaction container. The assignment step has a graph generation step of generating a graph having the primer pairs as vertices and non-specific amplification inducibility as an edge or a data structure equivalent to the graph, a coloring step of applying a solution to a graph coloring problem or the like to the graph to perform coloring such that the vertices adjacent to each other have different colors, and an association step of associating the plurality of colors with the reaction containers to associate the primer pair with the reaction containers of the corresponding colors.

    DATA PROCESSING METHOD, DATA PROCESSING APPARATUS, AND DATA PROCESSING PROGRAM

    公开(公告)号:US20240242780A1

    公开(公告)日:2024-07-18

    申请号:US18619978

    申请日:2024-03-28

    CPC classification number: G16B40/10 G16B40/20

    Abstract: The present disclosure enables to accurately extract a signal derived from a tumor cell from data in which a signal derived from a non-tumor cell is mixed. According to an aspect of the present invention, there is provided a data processing method executed by a data processing apparatus including a processor. The data processing method including causing the processor to execute: an input step of inputting first DNA profile data obtained by measuring a sample including a tumor cell and a plurality of known non-tumor cells; a signal removal step of removing a signal derived from the non-tumor cell, which is mixed in the input first DNA profile data, to acquire only a signal derived from the tumor cell; and an output step of outputting the signal derived from the tumor cell as a DNA profile feature amount of the sample.

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