公开(公告)号：US20150004695A1

公开(公告)日：2015-01-01

申请号：US14314873

申请日：2014-06-25

Applicant: FUJIFILM Corporation

Inventor： Naoyuki NISHIKAWA ,  Kyoko SENGA ,  Yuuki INOUE ,  Yuko IGARASHI

IPC: C12N15/113

CPC classification number: C12N15/111 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2320/32

Abstract: The present invention provides a lipid particle which has low cytotoxicity, can stably hold a nucleic acid molecule outside a cell (in blood), and can escape from an endosome and rapidly release the nucleic acid in the cytoplasm; a nucleic acid transfer carrier; a compound for manufacturing a nucleic acid transfer carrier; a method for manufacturing a lipid particle; and a gene transfer method. The lipid particle contains, as constituents, a phospholipid having one or more amino groups and one or more nitrogen-containing heterocyclic groups, a neutral lipid not containing a nitrogen-containing heterocyclic group, a sterol, and a nucleic acid.

Abstract translation: 本发明提供了具有低细胞毒性的脂质颗粒，可以将核酸分子稳定地保持在细胞外（血液中），并且可以从内体中逸出并迅速释放细胞质中的核酸; 核酸转运载体; 用于制造核酸转移载体的化合物; 一种制备脂质颗粒的方法; 和基因转移方法。 脂质颗粒含有具有一个或多个氨基和一个或多个含氮杂环基的磷脂，不含氮杂环基的中性脂质，固醇和核酸。