公开(公告)号：US20140107190A1

公开(公告)日：2014-04-17

申请号：US14118880

申请日：2012-05-21

申请人： Francisco Martin Molina ,  Karim Benabdellah El Khlanji ,  Marien Cobo Pulido ,  Miguel Garcia Toscano ,  Pilar Munoz Fernandez

发明人： Francisco Martin Molina ,  Karim Benabdellah El Khlanji ,  Marien Cobo Pulido ,  Miguel Garcia Toscano ,  Pilar Munoz Fernandez

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A01K2267/03 ,  A61K48/00 ,  C12N2740/15043 ,  C12N2830/005

摘要： The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.

摘要翻译： 本发明涉及用于基于原始TetR阻遏物的组成型表达的目的基因的调控表达的表达系统和由可操作地连接到四环素操纵子序列的操纵子序列的组成型启动子驱动的多核苷酸的表达。 该系统可以作为两种不同的多核苷酸或一体化载体提供。 本发明还涉及根据本发明的载体，宿主细胞和病毒颗粒以及其用于体外和体内产生感兴趣的产品或用于治疗的用途。

公开(公告)号：US09822379B2

公开(公告)日：2017-11-21

申请号：US14118880

申请日：2012-05-21

申请人： Francisco Martin Molina ,  Karim Benabdellah El Khlanji ,  Marien Cobo Pulido ,  Miguel Garcia Toscano ,  Pilar Munoz Fernandez

发明人： Francisco Martin Molina ,  Karim Benabdellah El Khlanji ,  Marien Cobo Pulido ,  Miguel Garcia Toscano ,  Pilar Munoz Fernandez

IPC分类号： C12N15/86 ,  C07H21/04 ,  C12P21/02 ,  A61K48/00

CPC分类号： C12N15/86 ,  A01K2267/03 ,  A61K48/00 ,  C12N2740/15043 ,  C12N2830/005

摘要： The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.