公开(公告)号：US11167028B2

公开(公告)日：2021-11-09

申请号：US16212245

申请日：2018-12-06

申请人： GLAXOSMITHKLINE BIOLOGICALS, S.A.

发明人： Luis Brito ,  Michelle Chan ,  Andrew Geall ,  Derek O'Hagan

IPC分类号： A61K39/39 ,  A61K9/107 ,  A61K47/06 ,  A61K47/24 ,  A61K39/12 ,  A61K39/155 ,  A61K39/245 ,  A61K47/02 ,  A61K47/12 ,  A61K47/22 ,  A61K47/26 ,  A61K47/34 ,  C12N7/00 ,  A61K39/00

摘要： This invention generally relates to cationic oil-in-water emulsions that contain high concentrations of cationic lipids and have a defined oil:lipid ratio. The cationic lipid can interact with the negatively charged molecule thereby anchoring the molecule to the emulsion particles. The cationic emulsions described herein are useful for delivering negatively charged molecules, such as nucleic acid molecules to cells, and for formulating nucleic acid-based vaccines.

公开(公告)号：US11135287B2

公开(公告)日：2021-10-05

申请号：US16295563

申请日：2019-03-07

申请人： GLAXOSMITHKLINE BIOLOGICALS, S.A.

发明人： Luis Brito ,  Andrew Geall ,  Derek O'Hagan ,  Manmohan Singh

IPC分类号： A61K39/395 ,  A61K39/385 ,  A61K39/39 ,  A61K9/107 ,  A61K39/00

摘要： This invention generally relates to cationic oil-in-water emulsions that can be used to deliver negatively charged molecules, such as an RNA molecule. The emulsion particles comprise an oil core and a cationic lipid. The cationic lipid can interact with the negatively charged molecule thereby anchoring the molecule to the emulsion particles. The cationic emulsions described herein are particularly suitable for delivering nucleic acid molecules (such as an RNA molecule encoding an antigen) to cells and formulating nucleic acid-based vaccines.

公开(公告)号：US11324770B2

公开(公告)日：2022-05-10

申请号：US16740924

申请日：2020-01-13

申请人： GLAXOSMITHKLINE BIOLOGICALS S.A.

发明人： Andrew Geall ,  Katrin Ramsauer ,  Gillis Otten ,  Christian Walter Mandl

IPC分类号： A61K31/7088 ,  A61K39/00 ,  A61K39/12

摘要： RNA encoding an immunogen is co-delivered to non-immune cells at the site of delivery and also to immune cells which infiltrate the site of delivery. The responses of these two cell types to the same delivered RNA lead to two different effects, which interact to produce a strong immune response against the immunogen. The non-immune cells translate the RNA and express the immunogen. Infiltrating immune cells respond to the RNA by expressing type I interferons and pro-inflammatory cytokines which produce a local adjuvant effect which acts on the immunogen-expressing non-immune cells to upregulate major histocompatibility complex expression, thereby increasing presentation of the translated protein to T cells. The effects on the immune and non-immune cells can be achieved by a single delivery of a single RNA e.g. by a single injection.

公开(公告)号：US11026890B2

公开(公告)日：2021-06-08

申请号：US16401452

申请日：2019-05-02

申请人： GLAXOSMITHKLINE BIOLOGICALS S.A.

发明人： Luis Brito ,  Michelle C. Archer ,  Andrew Geall ,  Derek O'Hagan ,  Manmohan Singh

IPC分类号： A61K9/107 ,  A61K31/713 ,  A61K39/12 ,  A61K39/155 ,  A61K9/00 ,  A61K39/00

摘要： This invention generally relates to cationic oil-in-water emulsions that can be used to deliver nucleic acid molecules, such as an RNA molecule. The emulsion particles comprise an oil core and a cationic lipid. The emulsion particles have an average diameter of about 80 nm to about 180 nm, and the emulsion have an N/P ratio of at least 1.1:1.

公开(公告)号：US20190091329A1

公开(公告)日：2019-03-28

申请号：US16212245

申请日：2018-12-06

申请人： GLAXOSMITHKLINE BIOLOGICALS, S.A.

发明人： Luis Brito ,  Michelle Chan ,  Andrew Geall ,  Derek O'Hagan

IPC分类号： A61K39/39 ,  A61K47/34 ,  A61K47/24 ,  A61K47/06 ,  A61K47/12 ,  A61K39/12 ,  C12N7/00 ,  A61K39/245 ,  A61K47/22 ,  A61K47/02 ,  A61K39/155 ,  A61K47/26 ,  A61K9/107 ,  A61K39/00

摘要： This invention generally relates to cationic oil-in-water emulsions that contain high concentrations of cationic lipids and have a defined oil:lipid ratio. The cationic lipid can interact with the negatively charged molecule thereby anchoring the molecule to the emulsion particles. The cationic emulsions described herein are useful for delivering negatively charged molecules, such as nucleic acid molecules to cells, and for formulating nucleic acid-based vaccines.