公开(公告)号：US11516979B2

公开(公告)日：2022-12-06

申请号：US16315016

申请日：2017-10-27

申请人： GUANGDONG PROVINCIAL BIOENGINEERING INSTITUTE (GUANGZHOU SUGARCANE INDUSTRY RESEARCH INSTITUTE)

发明人： Zhongxing Huang ,  Donglei Sun ,  Yuanxian Xu ,  Huiyi He ,  Lina Fan ,  Fangye Lao ,  Qiuping Ling ,  Yuxing An

IPC分类号： A01H1/06 ,  A01H1/00 ,  A01H4/00

摘要： The present invention relates to a method for conducting high-throughput and directed mutagenesis for sugarcane resistance to glyphosate by plasma. The method is as follows: sugarcane embryonic calli are irradiated by a plasma instrument under a sterile condition for mutagenesis, wherein the mutagenesis power is 140˜200 W, the discharging distance is 35˜45 mm, the mutagenesis time is 110˜140 s and the protective gas is nitrogen; buffering culture, moderate/high concentration of glyphosate stress screening, differentiation into seedlings, glyphosate stress screening of bottle seedlings and stress screening via spraying glyphosate on the leave surfaces of potted plants are conducted for the treated calli. The present invention has the advantages of safe operation, simplicity, practicability, high handling capacity, low contamination, and due to implementation of directed stress screening, high screening efficiency, decreased subsequent screening workload and visual identification of resistant mutant strains.