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公开(公告)号：US20240352449A1
公开(公告)日：2024-10-24
申请号：US18589331
申请日：2024-02-27
发明人： Ariel JAIMOVICH , Sven Jeffrey DUENWALD , Peter Vilem GRAUMAN , Yupeng HE , Charbel Said EID , Haley Dara AXELROD
IPC分类号： C12N15/10 , C12Q1/6886
CPC分类号： C12N15/1065 , C12Q1/6886 , C12Q2600/154 , C12Q2600/156
摘要： Disclosed herein are methods for isolating DNA, such as cell-free DNA (cfDNA) or DNA from a tissue sample, e.g., in which the DNA is partitioned into hypermethylated and hypomethylated partitions. After differential tagging of the partitions, portions of the hypomethylated partition are pooled with the hypermethylated partition or pooled separately. Epigenetic and sequence-variable target regions are captured from the pool comprising DNA from the hypermethylated and hypomethylated partitions, and sequence-variable target regions are captured from the pool comprising DNA from the hypomethylated partition. This approach can reduce costs and/or bandwidth by limiting sequencing of epigenetic target regions from the hypomethylated partition, which may be less informative than other DNA.
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公开(公告)号：US20220195419A1
公开(公告)日：2022-06-23
申请号：US17389199
申请日：2021-07-29
发明人： Ariel JAIMOVICH , Sven Jeffrey DUENWALD , Peter Vilem GRAUMAN , Yupeng HE , Charbel Said EID , Haley Dara AXELROD
IPC分类号： C12N15/10 , C12Q1/6886
摘要： Disclosed herein are methods for isolating DNA, such as cell-free DNA (cfDNA) or DNA from a tissue sample, e.g., in which the DNA is partitioned into hypermethylated and hypomethylated partitions. After differential tagging of the partitions, portions of the hypomethylated partition are pooled with the hypermethylated partition or pooled separately. Epigenetic and sequence-variable target regions are captured from the pool comprising DNA from the hypermethylated and hypomethylated partitions, and sequence-variable target regions are captured from the pool comprising DNA from the hypomethylated partition. This approach can reduce costs and/or bandwidth by limiting sequencing of epigenetic target regions from the hypomethylated partition, which may be less informative than other DNA.
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