专利检索 ap:("GUIZHOU MEDICAL UNIVERSITY" OR "MATERNAL AND CHILD HEALTH HOSPITAL OF GUIYANG CITY" OR "GUIZHOU ENTRY-EXIT INSPECTION AND QUARANTINE BUREAU") AND inv:"Hai Zhuang" 第 1 页

1.

发明授权
Kit for detecting ctDNA and expression of drug-resistant genes related to applicability of tumor chemotherapeutic drugs 有权

公开(公告)号：US10711313B2

公开(公告)日：2020-07-14

申请号：US15944725

申请日：2018-04-03

申请人： GUIZHOU MEDICAL UNIVERSITY , MATERNAL AND CHILD HEALTH HOSPITAL OF GUIYANG CITY , GUIZHOU ENTRY-EXIT INSPECTION AND QUARANTINE BUREAU

发明人： Wenfeng Yu , Shi Zhou , Zhi Huang , Bi Wang , Rui Gao , Dai Wei Zhao , Hai Zhuang , Ya Ling Liang

IPC分类号： C12Q1/68 , C07D227/02 , C12Q1/6886 , C12Q1/686 , C12Q1/6869

摘要： A kit for determining ctDNA concentration and a method for determining ctDNA concentration is disclosed, wherein the method comprising dissolving extracted ctDNA in Tris-HCl buffer with pH>7.0 and adding into the buffer 2,7-bis(1-methyl-4-vinylpyridine)-9-ethylcarbazole iodised salt as a molecular probe, calculating the concentration of ctDNA in the solution by measuring the Fluorescence intensity of the solution. It has extremely high sensitivity in the determination of 0 μg/ml˜50 μg/ml ctDNA solution.