公开(公告)号：US20210032681A1

公开(公告)日：2021-02-04

申请号：US17028367

申请日：2020-09-22

Applicant: Gen-Probe Incorporated

Inventor： David BUSE ,  David Howard COMBS ,  Nobert D. HAGEN ,  David OPALSKY ,  Bruce RICHARDSON ,  Anita PRASAD ,  Keith MORAVICK ,  Tyler MOORE ,  Byron J. KNIGHT

IPC: C12Q1/686 ,  B01L7/00 ,  G01N21/64

Abstract: Multiple receptacles containing a reaction mixture are transported to corresponding receptacle wells of a thermally-conductive receptacle holder in thermal communication with a support that is in thermal communication with a heat sink. The heat sink is pre-heated to a temperature above ambient temperature, and the temperature of the receptacle holder is cycled. Optical communication is established between each of the receptacle wells and an excitation signal source and an emission signal detector during cycling, and whether an emission signal is emitted from any of the receptacles is determined as an indication of the presence of a target nucleic acid.