公开(公告)号：US09862778B2

公开(公告)日：2018-01-09

申请号：US14251428

申请日：2014-04-11

Applicant: Genentech, Inc.

Inventor： Glen Giese ,  Amy Lim ,  Josefine Persson ,  Justin Scheer ,  Ambrose Williams

IPC: C07K16/46

CPC classification number: C07K16/468 ,  C07K2317/31 ,  C07K2317/522 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/53 ,  C07K2317/56 ,  C07K2317/622

Abstract: Described herein are methods for the efficient production of a heteromultimeric protein, such as a bispecific antibody. Heteromultimeric proteins may be capable of specifically binding more than one target molecule or different epitopes on a single target molecule. The methods modulate parameters to improve assembly of the heteromultimeric proteins at higher yield and efficiency than otherwise possible. Also described are compositions comprising a hinge-containing polypeptide, such as a half-antibody.

公开(公告)号：US11725065B2

公开(公告)日：2023-08-15

申请号：US16820660

申请日：2020-03-16

Applicant: Genentech, Inc.

Inventor： Glen Giese ,  Amy Lim ,  Josefine Persson ,  Justin Scheer ,  Ambrose Williams

IPC: C07K16/46

CPC classification number: C07K16/468 ,  C07K2317/31 ,  C07K2317/522 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/53 ,  C07K2317/56 ,  C07K2317/622

Abstract: Described herein are methods for the efficient production of a heteromultimeric protein, such as a bispecific antibody. Heteromultimeric proteins may be capable of specifically binding to more than one target molecule or different epitopes on a single target molecule. The methods modulate parameters to improve assembly of the heteromultimeric proteins at higher yield and efficiency than otherwise possible. Also described are compositions comprising a hinge-containing polypeptide, such as a half-antibody.