公开(公告)号：US20130281314A1

公开(公告)日：2013-10-24

申请号：US13829094

申请日：2013-03-14

Applicant: Genentech, Inc.

Inventor： Jacob E. CORN ,  Yingnan ZHANG ,  Aaron H. PHILLIPS

IPC: C12Q1/37

CPC classification number: C12Q1/37 ,  C07K14/00 ,  G01N2500/04

Abstract: Provided herein are conformationally stabilized ubiquitin proteins and methods for using the same to identify agents that bind to the stabilized ubiquitin protein or that bind to a protein that interacts with or processes the stabilized form of the ubiquitin protein. Also provided herein are methods for screening for conformationally stabilized proteins having increased binding affinity to a binding partner in comparison to the binding affinity of the wildtype form of the protein to the binding partner.

Abstract translation: 本文提供了构象稳定的泛素蛋白质及其使用方法，用于鉴定与稳定的泛蛋白结合的试剂或结合与泛素蛋白质的稳定化形式相互作用或处理的蛋白质。 本文还提供了与蛋白质的野生型形式与结合配偶体的结合亲和力相比较，筛选具有增加的结合亲和力的结构亲和力的构象稳定的蛋白质的方法。