公开(公告)号：US20170283867A1

公开(公告)日：2017-10-05

申请号：US15085713

申请日：2016-03-30

Applicant: Genia Technologies, Inc.

Inventor： Ashraf Wahba ,  William Nielsen ,  J. William Maney, JR. ,  Kevin Aliado ,  Kyle Umeda ,  Roger J.A. Chen ,  Jing Luo

IPC: C12Q1/68

Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US20170283866A1

公开(公告)日：2017-10-05

申请号：US15085700

申请日：2016-03-30

Applicant: Genia Technologies, Inc.

Inventor： Ashraf Wahba ,  William Nielsen ,  Jing Luo ,  Kevin Aliado ,  Kyle Umeda ,  Roger J.A. Chen

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/607

Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.