专利检索 ap:("Gerold Schuler" OR "Zwi N. Berneman" OR "Viggo F. I. Van Tendeloo" OR "Peter Ponsaerts" OR "Isolde Strobel") AND inv:"Peter Ponsaerts" 第 1 页

1.

发明授权
Transfection of eukaryontic cells with linear polynucleotides by electroporation 有权
标题翻译：通过电穿孔用线性多核苷酸转染真核细胞

公开(公告)号：US07547551B2

公开(公告)日：2009-06-16

申请号：US10177390

申请日：2002-06-20

申请人： Gerold Schuler , Zwi N. Berneman , Viggo F. I. Van Tendeloo , Peter Ponsaerts , Isolde Strobel

发明人： Gerold Schuler , Zwi N. Berneman , Viggo F. I. Van Tendeloo , Peter Ponsaerts , Isolde Strobel

IPC分类号： C12N15/87

CPC分类号： C12N15/87

摘要： The present invention provides an improved method for gene delivery in eukaryotic cells by electroporation, preferably in human hematopoietic cells, particular dendritic cells. The method of the invention is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells.

摘要翻译： 本发明提供了一种用于真核细胞中通过电穿孔，优选在人类造血细胞，特定树突细胞中的基因递送的改进方法。本发明的方法优于mRNA的脂质转染和被动脉冲和用于基因递送的质粒cDNA的电穿孔，包括树突状细胞的肿瘤抗原加载。