公开(公告)号：US5244655A

公开(公告)日：1993-09-14

申请号：US822092

申请日：1992-01-17

申请人： Giuseppe C. Viscomi ,  Paolo Rappuoli

发明人： Giuseppe C. Viscomi ,  Paolo Rappuoli

IPC分类号： A61K38/00 ,  C07K14/565 ,  C12N15/22

CPC分类号： C07K14/565 ,  A61K38/00

摘要： A process is described for the purification of recombinant human beta interferon which comprises the use of three chromatographic stages carried out in series, utilizing as stationary phases: glass particles of controlled porosity, cation exchange polymer resins and polymer resins able to complex heavy metals.

摘要翻译： 描述了用于纯化重组人β干扰素的方法，该方法包括使用串联进行的三个色谱步骤：利用可控孔隙的玻璃颗粒，阳离子交换聚合物树脂和能够使重金属络合的聚合物树脂。

公开(公告)号：US5066786A

公开(公告)日：1991-11-19

申请号：US220312

申请日：1988-07-18

申请人： Otello Protasi ,  Paolo Rappuoli

发明人： Otello Protasi ,  Paolo Rappuoli

IPC分类号： A61K38/00 ,  C07K14/565

CPC分类号： C07K14/565 ,  A61K38/00

摘要： A method is described for the purification of crude human interferon from solutions containing it, which comprises:a) the complete adsorption of the crude interferon in a column of siliceous material which has previously been disinfected with an aqueous solution of formaldehyde;b) the washing of the column with non-pyrogenic, sterile, deionized water;c) the removal of the extraneous residual proteins by the elution of the column successively with a 1.4 M aqueous solution of NaCl in non-pyrogenic, sterile, deionized water, and with an aqueous solution of acetic acid having a molar concentration of 0.001 M to 0.003 M;d) the elution of the interferon from the column with an aqueous solution of acetic acid having a molar concentration of from 0.01 to 0.03 M and finally,e) the recovery and lyophilization of the elution containing the purified interferon.The method enables interferon to be obtained, the purity of which, measured as its specific activity, is at least 1000 times greater than that of the crude interferon, with a yield greater than 85%.The interferon thus purified is particularly useful for treatment in man.