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公开(公告)号:US09291596B2
公开(公告)日:2016-03-22
申请号:US13764934
申请日:2013-02-12
IPC分类号: G01N27/447
CPC分类号: G01N27/44747 , G01N27/44739
摘要: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.
摘要翻译: 用于将蛋白质或多肽从聚丙烯酰胺凝胶快速转移到合适的膜如硝酸纤维素或聚偏二氟乙烯(PVDF)的半干式一步电泳转移缓冲液组合物。 该组合物含有最小化电阻的组分,并且能够使用常规容易获得的滤纸即棉纤维素纤维实现高效快速半干转印。