1,3-dicarbonyl-dioxygenase—a new c-c-bond cleaving enzyme
    1.
    发明授权
    1,3-dicarbonyl-dioxygenase—a new c-c-bond cleaving enzyme 失效
    1,3-二羰基双加氧酶 - 一种新的c-c键切割酶

    公开(公告)号:US06790644B2

    公开(公告)日:2004-09-14

    申请号:US10141225

    申请日:2002-05-07

    IPC分类号: C12N902

    CPC分类号: C12N9/0069

    摘要: An enzyme that cleaves 1,3-dicarbonyl compounds by incorporating molecular oxygen into the substrate in amounts equimolar to the amount of substrate cleaved. The enzyme from Acinetobacter johnsonii is a multimer of about 67 kilodaltons made up of subunits of about 16.6 kilodaltons. Nucleotide and amino acid sequence analysis of a cloned A. johnsonii subunit indicates little homology with other proteins. Native or recombinant enzyme can be used to decontaminate or detoxify acetylacetone or related diketones.

    摘要翻译: 通过将分子氧引入底物中的量与切割的底物的量相等的量来切割1,3-二羰基化合物的酶。 来自不动杆菌属的不动杆菌属的酶是由约16.6千道尔顿的亚基组成的约67千道尔顿的多聚体。 克隆的约翰逊亚单位的核苷酸和氨基酸序列分析表明与其他蛋白质几乎没有同源性。 天然或重组酶可用于消除乙酰丙酮或相关二酮的去污或解毒。