公开(公告)号：US06682733B1

公开(公告)日：2004-01-27

申请号：US08653104

申请日：1996-05-24

申请人： Gunnar Pohl ,  Lennart Hansson ,  Björn Löwenadler

发明人： Gunnar Pohl ,  Lennart Hansson ,  Björn Löwenadler

IPC分类号： A61K3848

CPC分类号： C12N9/6459 ,  A61K2123/00 ,  C12Y304/21069

摘要： A fibrinolytically active plasminogen activator of the tissue type, wherein the growth factor (G) domain has been deleted, and wherein also the K1 domain has been deleted and additionally has been modified in one or more of the following sites or region: the sites of amino acid residues 177, 184, 277 and 448 and the F domain, the F domain modification if present being a deletion of part or all of said domain; DNA-sequence comprising a nucleotide sequence coding for said plasminogen activator; expression vector which in a transformed host cell can express said DNA-sequence; host cell transformed using such vector; pharmaceutical composition comprising the fibrinolytically active plasminogen activator; plasminogen activator for use in treating thrombotic disease; a process for the manufacture of such fibrinolytically active plasminogen activator; a process for the treatment of thrombotic disorder; and a process of localizing thrombi while using such plasminogen activator.

摘要翻译： 组织类型的纤维蛋白溶解活性纤溶酶原激活剂，其中生长因子（G）结构域已经被缺失，并且其中K1结构域已经被删除并且另外已经在以下一个或多个位点或区域被修饰： 氨基酸残基177,184,277和448以及F结构域，F域修饰如果存在的话是部分或全部所述结构域的缺失;包含编码所述纤溶酶原激活物的核苷酸序列的DNA序列; 转化的宿主细胞可以表达所述DNA序列;使用这种载体转化的宿主细胞;包含纤维蛋白溶解活性纤溶酶原激活剂的药物组合物;用于治疗血栓形成疾病的纤溶酶原激活剂;制备这种纤维蛋白溶解活性纤溶酶原激活剂的方法; 治疗血栓性疾病; 以及使用这种纤溶酶原激活剂定位血栓的过程。