公开(公告)号：US20240150716A1

公开(公告)日：2024-05-09

申请号：US18550232

申请日：2022-03-18

Applicant: HEMERA S.R.L. ,  HUMANITAS MIRASOLE S.P.A.

Inventor： Ilaria DECIMO ,  Francesco BIFARI ,  Sissi DOLCI ,  Guido Francesco FUMAGALLI ,  Massimo LOCATI

IPC: C12N5/0786 ,  A61K35/15 ,  A61P25/00

CPC classification number: C12N5/0645 ,  A61K35/15 ,  A61P25/00 ,  C12N2500/02 ,  C12N2501/22 ,  C12N2502/30

Abstract: The present invention relates to an in vitro or ex vivo method for inducing a phenotypic and/or functional change in a population of mononuclear phagocytes isolated from biological samples. The method includes the incubation of the population in a culture medium which includes factors released from tumor cultures or explants. The incubation takes place under hypoxic conditions and the incubation induces a phenotypic and/or functional change in the mononuclear phagocytes of the population. The macrophages thus obtained assume a unique regenerative phenotype not present in other polarized phenotypes, including M2 macrophages. In addition, the invention relates to the use of the bioreactor thus produced for the regeneration of tissues, including neural tissue.