公开(公告)号：US20200040374A1

公开(公告)日：2020-02-06

申请号：US16298789

申请日：2019-03-11

Applicant: HITACHI, LTD.

Inventor： Chihiro MANRI ,  Hideyuki NODA ,  Naoko SENDA

IPC: C12Q1/06 ,  C12Q1/66

Abstract: Provided is a method for detecting a microorganism in a test sample containing cells, including a step of adding the sample to a microorganism culture medium, a step of culturing the microorganism culture medium containing the sample, a step of sampling a part of the culture medium at a predetermined time, a step of acquiring the ATP level of the sampled culture medium, and a step of detecting the microorganism in the sample based on the change of the ATP level over time.

公开(公告)号：US20250060334A1

公开(公告)日：2025-02-20

申请号：US18721968

申请日：2022-12-08

Applicant: HITACHI, LTD.

Inventor： Chihiro MANRI ,  Takahide YOKOI ,  Takahiro ANDO

IPC: G01N27/447 ,  C12Q1/6853 ,  C12Q1/6858

Abstract: Provided is a method and a means for improving multiplex simultaneous detection of a structural polymorphism mutation in capillary electrophoresis. In one embodiment, the present invention relates to a method for detecting a mutation in a test nucleic acid, the method including: providing a first primer set, containing at least one labeled first primer, for amplifying a nucleic acid with a first mutation; providing a second primer set, containing at least one labeled second primer, for amplifying a nucleic acid with a second mutation; amplifying the test nucleic acid with use of the first primer set and the second primer set; and subjecting an obtained amplification product to capillary electrophoresis to analyze the mutation in the test nucleic acid, the first primer and the second primer being individually labeled with combinations of different numbers and/or kinds of fluorescent dyes.