公开(公告)号：US20230134376A1

公开(公告)日：2023-05-04

申请号：US17918097

申请日：2021-04-07

Applicant: HUMANITAS MIRASOLE S.P.A.

Inventor： Andrea DONI ,  Diego MORONE

IPC: A61B5/1455 ,  A61B5/145

Abstract: This invention pertains to a fluorescence lifetime imaging microscopy (FLIM)-based method for the quantitative three-dimensional (3D) imaging of the metabolic status of a lesion, including the acquisition of FLIM data of a least one anaerobic glycolysis marker. This method applies in particular to lesions such as wounds and tumours, and to markers such as the NADH/NAD+ ratio, the NAD(P)H/NAD+ ratio, the NADH/FAD ratio, the NAD(P)H/FAD ratio or the FADH/FAD ratio as a first marker, and their optional combination with a second marker such as pH, oxygen tension, glucose, pyruvate and lactate.