公开(公告)号：US6132988A

公开(公告)日：2000-10-17

申请号：US738168

申请日：1996-10-25

申请人： Hiromu Sugino ,  Takanori Nakamura ,  Hiroki Shouji

发明人： Hiromu Sugino ,  Takanori Nakamura ,  Hiroki Shouji

IPC分类号： C07K14/71 ,  C12N15/12 ,  C12N15/63 ,  C12N15/85

CPC分类号： C07K14/71

摘要： To provide a method of isolating and detecting a new receptor gene, as a means of elucidating the function of neuronal cell-specific receptors, especially of elucidating the detailed mechanism of the neuronal cell differentiation inhibitory and nerve nutrition factor-like actions of activin receptors, DNA containing said new receptor gene, a method of producing a protein encoded by this new receptor gene, and use for this DNA and protein. The receptor protein of the present invention and DNA encoding this protein can be used for various purposes, including 1) ligand determination, 2) obtainment of antibodies and antisera, 3) construction of recombinant receptor protein expression systems, 4) development of receptor binding assay systems and screening for pharmaceutical candidate compounds using expression systems, 5) drug designing based on comparison with structurally similar ligand receptors, 6) reagent for preparation of probes and PCR primers for gene diagnosis, and 7) drug for gene therapy.

摘要翻译： 为了提供分离和检测新受体基因的方法，作为阐明神经元细胞特异性受体功能的手段，特别是阐明神经元细胞分化抑制和激活素受体的神经营养因子样作用的详细机制， 含有所述新受体基因的DNA，由该新受体基因编码的蛋白质的制备方法，以及该DNA和蛋白质的用途。 本发明的受体蛋白质和编码该蛋白质的DNA可用于各种目的，包括1）配体测定，2）抗体和抗血清的获得，3）重组受体蛋白表达系统的构建，4）受体结合试验的发展 系统和使用表达系统筛选药物候选化合物，5）基于与结构相似的配体受体比较的药物设计，6）用于基因诊断的探针和PCR引物的制备试剂和7）用于基因治疗的药物。