Chemical sensors
    2.
    发明申请
    Chemical sensors 审中-公开
    化学传感器

    公开(公告)号:US20080070794A1

    公开(公告)日:2008-03-20

    申请号:US11804905

    申请日:2007-05-21

    IPC分类号: C40B30/04 C40B70/00

    摘要: In one embodiment the invention provides a mutant UDP-glucose receptor (P2Y14) functionally expressed in the yeast Saccharomyces. The mutant receptors have ligand-binding properties that are useful as practical biosensors. Mutagenesis of the entire UDP-glucose receptor gene yielded receptors with increased activity but similar ligand specificities, while random mutagenesis of residues in the immediate vicinity of the ligand-binding pocket yielded mutants with altered ligand specificity. The receptor mutants can be used to detect chemical ligands in complex mixtures and to discriminate among chemically or stereochemically related compounds. Also provided are methods for combinatorial applications wherein engineered receptors can be applied, for example, in a pairwise manner to differentiate among several chemical analytes that would be indistinguishable with a single receptor.

    摘要翻译: 在一个实施方案中,本发明提供了在酵母酵母中功能性表达的突变UDP-葡萄糖受体(P2Y14)。 突变体受体具有可用作实际生物传感器的配体结合特性。 整个UDP-葡萄糖受体基因的诱变产生具有增加的活性但相似的配体特异性的受体,而在配体结合口袋附近随机诱变残基产生具有改变的配体特异性的突变体。 受体突变体可用于检测复杂混合物中的化学配体,并区分化学或立体化学相关化合物。 还提供了用于组合应用的方法,其中工程化受体可以例如以成对方式应用于区分几种与单一受体无法区分的化学分析物。