公开(公告)号：US10418126B2

公开(公告)日：2019-09-17

申请号：US15061130

申请日：2016-03-04

申请人： Hoffmann-La Roche Inc.

发明人： Hubert Kettenberger ,  Stefan Klostermann ,  Florian Lipsmeier ,  Apollon Papadimitriou ,  Jasmin Sydow-Andersen

IPC分类号： G16B15/00 ,  G01N33/68 ,  C07K16/10 ,  C07K16/22 ,  G16C99/00

摘要： Herein is reported a method for selecting or deselecting an antibody comprising a) determining for each Asp and Asn residue in an antibody Fv region the conformational flexibility of the Cα-atom using a homology model ensemble, b) determining for each Asp and Asn residue in an antibody Fv region the size of the amino acid residue C-terminal to the Asp or Asn residue, and c) selecting an antibody in which the Cα-atom is conformationally inflexible and/or the Asp or Asn has a big C-terminal amino acid residue, or deselecting an antibody in which the Cα-atom has a moderate to high conformational flexibility and/or the Asp or Asn has a small C-terminal amino acid residue.